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220C MRB University of Kansas Lawrence, KS 66045 Phone: (785) 864-3072 Fax: (785) 864-1916 Email: clunte@ku.edu |
Monitoring Chemistry in Living Organisms
Research is focused on the development of new methods for the study of drug metabolism and disposition. All aspects of the bioanalytical problem of drug disposition studies are being addressed, from sampling of living organisms to analysis of minute biological samples. A major focus is the development of microdialysis sampling in vivo. The research group has provided both novel applications of microdialysis sampling and fundamental studies of the technique. Several novel microdialysis probes have been developed for sampling from peripheral tissue in conscious animals. Studies of drug transport effects on the microdialysis process in vivo have led to greater insight into the use of this technique.
Research also involves the development of microanalytical techniques based on separations. On-column sample concentration methods, electrochemical detectors and mass spectrometer interfaces for capillary electrophoresis (CE) are being developed. These microanalytical techniques are being directly coupled to microdialysis sampling to provide separation-based in vivo sensors. These systems provide near real-time monitoring of multiple chemical species in the tissues of awake freely-moving animals.
These new bioanalytical tools are being applied to several important problems in drug metabolism and disposition studies. Some current projects include studying the neuropharmacology of drugs to fight addiction, a study on the adsorption of pharmaceuticals in healthy and ulcerated stomachs, an investigation of the transdermal delivery of therapeutic agents, and approaches to inhibiting epileptic seizures. The group has recently embarked on a new project to develop analytical methods to detect biomarkers for oxidative DNA damage and lipid peroxidation occurring as a result of oxidative stress during heart attack and stroke. These methods will then be used with microdialysis sampling in the heart and brain to study the affects of oxidative stress during heart attack and stroke.
pH-Mediated Acid Stacking with Reverse Pressure for the Analysis of Cationic Pharmaceuticals in Capillary Electrophoresis. J.A. Gillogly and C.E. Lunte, Electrophoresis 26(2005)633-639.
Concentration Profiling in Rat Tissue by High-Resolution Magic Angle Spinning (HR-MAS) NMR Spectroscopy: Investigation of a Model Drug. L.H. Lucas, S.F. Wilson, C.E. Lunte, and C.K. Larive, Anal. Chem. 77(2005)2978-2984.
Tissue Targeted Metabonomics: Metabolic Profiling by Microdialysis Sampling and Microcoil NMR. K.E. Price, S.S. Vandaveer, C.E. Lunte, and C.K. Larive, J. Pharm. Biomed. Anal. 38(2005)904-909.
Determination of 8-Oxoguanine and 8-Hydroxydeoxyguanosine in the Rat Cerbral Cortex Using Microdialysis Sampling and Capillary Electrophoresis with Electrochemical Detection. S.D. Arnett, D.M. Osbourn, K.D. Moore, S.S. Vandaveer, and C.E. Lunte, J. Chromatogr. B 827(2005)16-25.
On-Column Preconcentration of Glutathione and Glutathione Disulfide Using Ph-Mediated Base Stacking for the Analysis of Microdialysis Samples by Capillary Electrophoresis. M.E. Hoque, S.D. Arnett, and C.E. Lunte, J. Chromatogr. B 827(2005)51-57.
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