Title: Graduate Student
Email: pradyot@ku.edu
Microdialysis sampling has been shown to be a powerful technique for pharmacokinetic and neurochemical investigations. Analytes diffuse across dialysis membrane based on concentration gradient. Slow flow rates are generally preferred, especially for trace analytes , due to higher analyte recoveries across the probe. Provides a protein-free sample. Both on-line or off-line analysis can be performed. Capillary electrophoresis (CE) is an ideal technique for analysis of dialysates . CE requires small sample and reagent volumes – helps in achieving better temporal resolution compared to LC, which has higher sample volume requirement. LC requires discrete samples whereas microdialysis is a continuous sampling technique. Coupling microdialysis to microchip CE is an alternative to conventional CE systems.
Microchip platforms have small sample and reagent requirements, fast analysis times, allowing improved temporal resolution. Sample preparation and derivatization can be achieved on the planar chip format, minimizing the number of external connections required. Microchips can be mass produced by lithographic methods. Microdialysis can also be used as the sample introduction technique for analysis on chip. The objectives of this research are to develop a separation-based sensor for aspartate and glutamate. Online derivatization of Asp and Glu will be achieved using naphthalene 2 ,3 dicarboxaldehyde (NDA)/ cyanide(CN-). The device will be used to monitor the concentrations of Asp and Glu in brain dialysate samples using a microdialysis – microchip CE system.
