Title: Post Doctoral Researcher
Email: mhoque@ku.edu
Web link: http://www.people.ku.edu/~mhoque
I work on a project to study biomarkers of oxidative stress using in vivo microdialysis and separation technique. Oxidative stress results from an imbalance between the generation and removal of reactive oxygen species (ROS) in biological systems causing an excess of ROS or a decrease in antioxidant concentration. ROS react with biomolecules resulting lipid peroxidation and degradation of DNA, proteins, and carbohydrates. ROS and its reaction products with biomolecules could be useful biomarkers of oxidative stress. Hence, it is necessary to develop appropriate analytical methods and experimental protocols to accurately measure and continuously monitor the concentration levels of biomarkers of oxidative stress in biological samples.
In this project, potential biomarkers, e.g. superoxide (O2.-), hydroxyl radical (.OH), glutathione (GSH), glutathione disulfide (GSSG), malondialdehyde (MDA), and 8-hydroxy-2-deoxyguanosine (8-OHdG), are being studied. Reactive oxygen species (i.e. O2.- and .OH) are formed in biological systems through normal metabolic processes and during oxidative stress. GSH (a tripeptide of glutamic acid, cysteine, and glycine) is an endogenous antioxidant, and GSSG is the oxidation product of GSH. MDA is an end product of lipid peroxidation, and 8-OHdG is a product of oxidative DNA damage. I have been working on three biomarkers, e.g. GSH, GSSG, and .OH. The purpose of my work is to develop analytical methods based on separations and utilize the method to monitor the changes in biomarkers’ levels in biological samples to estimate the extent of oxidative stress during oxidative events, e.g. ischemia and reperfusion. The outcome of this research will help to better understand the antioxidant activities, assess the extent of cellular damage, and find out any therapeutic routes.
In my research, microdialysis sampling technique is being used. Biological samples, collected from extra-cellular spaces of the targeted tissue sites of living or anesthetized animals through in vivo microdialysis sampling, give tissue specific chemical information. To analyze microdialysis samples, I use a separation technique. Different separation techniques, e.g. HPLC and CE, have different sample volume requirements. HPLC requires a sample volume in the microliter range (10 to 40 mL). However, CE requires only a small volume of sample (~nL), and would therefore be the better choice to couple to microdialysis sampling since it produces microlitervolume sample (e.g. 1mL/min). CE has proven to be a useful analytical tool for the analysis of volume-limited biological samples, such as microdialysates and single cells. In this study, CE with UV and EC detection is being used for continuous monitoring of biomarkers in microdialysis samples for good temporal resolution.
I was born and raised in Bangladesh, a small country in south Asia. I moved to USA for pursuing higher studies in the field of Chemistry in January of 1999.
I am married with Fauzia Mustafa since January, 2002. She has B.Sc. and M.Sc. degrees in Zoology from Bangladesh. She is planning to go school soon here in USA for pursuing higher studies in the field of Microbiology.
I like traveling new places, watching movies, having good times with the people I like. Also, I like listening to music, watching games, and playing tennis and ping pong.
