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1251 Wescoe Hall Drive Malott Hall, Room 4023 University of Kansas Lawrence, KS 66045 Phone: (785) 864-2971 Fax: (785) 864-5396 Email: jlimburg@ku.edu |
Bioinorganic Chemistry
The chemistry and biochemistry of metalloenzymes.
Bioinorganic chemistry lies at the interface of inorganic chemistry and biochemistry, focusing on the study of metalloproteins. We are particularly interested in metalloproteins that catalyze oxidation reactions, especially those that use iron as a cofactor. Such enzymes are capable of activating O2 into highly reactive intermediates, and then carefully directing the chemistry of these species. These intermediates are highly unstable, and for this reason they are difficult to study. We will develop artificial ways of producing reactive intermediates under conditions where they are long-lived and open to spectroscopic and structural characterization. This will open the door to understanding how enzymes have evolved specific structures to control these oxidation reactions. We are also interested in exploiting the reactivity, selectivity and specificity of metalloenzymes to develop biocatalysts. Directed evolution can be used to extend the capability of an enzyme towards reactivity with non-natural substrates. This allows an enzyme to be customized for a specific application, and we will use this methodology to produce environmentally benign catalysts.
Julian Limburg, John S. Vrettos, Louise M. Liable-Sands, Arnold L. Rheingold, Robert H.
Crabtree and Gary W. Brudvig, A Functional Model for O-O Bond Formation by the O2-Evolving
Complex in Photosystem II, (1999) Science 283, 1524-1527.
Julian Limburg, Veronika A. Szalai and Gary W. Brudvig, A Mechanistic and Structural Model for the Formation and Reactivity of a MnV=O Species in Photosynthetic Water Oxidation, (1999) J. Chem. Soc., Dalton Trans. 1353-1363.
Julian Limburg, John S. Vrettos, Hongyu Chen, Julio C. de Paula, Robert H. Crabtree and Gary W. Brudvig, “Characterization of the O2-Evolving Reaction Catalyzed by [(terpy)(H2O)MnIII(O)2MnIV(OH2)(terpy)](NO3)3 (terpy = 2,2’:6,2”-terpyridine)", (2001) J. Am. Chem. Soc. 123, 423-430.
