Neurons communicate by the release of neurotransmitters at the pre-synaptic terminal, a process called exocytosis. Immediately following exocytosis, released neurotransmitter is actively taken back up into the neuron by transporters. A thorough investigation of neurotransmitter release and uptake requires the use of techniques having adequate sensitivity to detect the analyte of interest and sufficient temporal resolution to distinguish between the release and uptake processes. The goal of our research program is the development and application of bioanalytical techniques capable of studying this signaling process. A wide array of techniques will be employed, including fluorescence microscopy, biochemical methods, and state-of-the-art electrochemical techniques which allow for the monitoring of biogenic molecules on physiologically relevant time scales. These techniques will be used to study a variety of important problems, including neurodegeneration, oxidative stress, and mechanisms of drug action.
